Biography

Education

  • 04/2019 – 03/2022 | Ph.D. (Pharmaceutical Sciences)
    Department of Experimental Genome Research, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
  • 04/2017– 03/2022 | Leading program (Medical Sciences)
    Interdisciplinary Program for Biomedical Sciences, Osaka University, Osaka, Japan
  • 04/2017– 03/2019 | Master of Science Degree (Pharmaceutical Sciences)
    Department of Experimental Genome Research, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan
  • 04/2013– 03/2017 | Bachelor’s Degree (Pharmaceutical Sciences; salutatorian)
    Department of Pharmaceutical Sciences, Osaka University, Osaka, Japan

Professional History

  • 07/2022 –  Present | Post-doctoral research fellow
    Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX
  • 04/2022 – 06/2022 | Post-doctoral research fellow
    Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
  • 04/2019 – 03/2022 | Pre-doctoral research fellow
    The Japan Society for the Promotion of Science (JSPS) research fellow

Honors and Awards

  • 2022 Osaka University, The excellent dissertation award
  • 2019 Japan Student Services Organization, The excellent academic award
  • 2018 Interdisciplinary Program for Biomedical Sciences, The best poster award
  • 2017 Osaka University, The Department Award for Academic Excellence
  • 2016 Ono Scholarship, The excellent academic award
  • 2015 Osaka University, The Dean Award for Academic Excellence

Fellowships & Grants

  • 07/2023 – 06/2025 | The Japan Society for the Promotion of Science
    Overseas Research Fellowships
    Title: In vitro reconstitution and analysis of germ cell differentiation process using formative pluripotent stem cells
  • 07/2022– 06/2023 | The Naito Foundation
    Title: In vitro gametogenesis using formative stem cells
  • 04/2020 – 03/2021 | Interdisciplinary Program for Biomedical Sciences
    Grant-in-Aid for Interdisciplinary Research
    Title: Development of AI staging system for mouse seminiferous tubule cross-sections using convolutional neural network
  • 04/2019 – 03/2022 | The Japan Society for the Promotion of Science
    Research Fellowship for Young Scientists
    Project number: 19J21619
    Title: Development of novel CRISPR/Cas9-mediated mutation repair

Publication

Original articles

  1. Ballard E, Sakurai M, Yu L, Liu L, Oura S, Huang J, Wu J. Incompatibility in cell adhesion constitutes a barrier to interspecies chimerism. Cell Stem Cell. 2024 Aug 18:S1934-5909(24)00286-8.
  2. Wang H, Kobayashi H, Shimada K, Oura S, Oyama Y, Kitakaze H, Noda T, Yabuta N, Miyata H, Ikawa M. MYCBPAP is a central apparatus protein required for centrosome-nuclear envelope docking and sperm tail biogenesis in mice. J. Cell. Sci. 2024 Aug 2:jcs.261962. doi: 10.1242/jcs.261962.
  3. Noda T, Shinohara H, Kobayashi S, Taira A, Oura S, Duritahala, Tokuyasu M, Araki K, Ikawa M. Multiple genes in the Pate5-13 genomic region contribute to ADAM3 processing. Biol Reprod. 2024 Apr 11;110(4):750-760.
  4. Yu L, Logsdon D, Pinzon-Arteaga CA, Duan J, Ezashi T, Wei Y, Ribeiro Orsi AE, Oura S, Liu L, Wang L, Liu K, Ding X, Zhan L, Zhang J, Nahar A, Stobbe C, Katz-Jaffe M, Schoolcraft WB, Tan T, Hon GC, Yuan Y, Wu J. Large-scale production of human blastoids amenable to modeling blastocyst development and maternal-fetal cross talk. Cell Stem Cell. 2023 Sep 7;30(9):1246-1261.e9.
  5. Liu L, Oura S, Markham Z, Hamilton JN, Skory RM, Li L, Sakurai M, Wang L, Pinzon-Arteaga CA, Plachta N, Hon GC, Wu J. Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids. Cell. 2023 Aug 31;186(18):3776-3792.e16.
  6. Kiyozumi D, Shimada K, Chalick M, Emori C, Kodani M, Oura S, Noda T, Endo T, Matzuk MM, Wreschner DH, Ikawa M. A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility. Nat Commun. 2023 Apr 24;14(1):2354.
  7. Shimada K, Park S, Oura S, Noda T, Morohoshi A, Matzuk MM, Ikawa M. TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2221762120.
  8. Kaneda Y, Miyata H, Shimada K, Oura S, Ikawa M. Testis-specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice. Andrology. in press
  9. Kuribayashi S, Fukuhara S, Tsujimura G, Imanaka T, Okada K, Ueda N, Takezawa K, Kiuchi H, Saito S, Takahashi Y, Kioka H, Oura S, Shimada K, Ikawa M, Nonomura N. Evaluation of the efficacy of creatine chemical exchange saturation transfer imaging in assessing testicular maturity. Reprod Med Biol. 2023 Feb 23;22(1):e12507.
  10. Oura S, Ninomiya A, Satoh T, Sugihara F, Koyano T, Matzuk MM, Ikawa M. Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci Rep. 2022 Dec 23; 12(1):22198.
  11. Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, Ikawa M. Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLoS Genet. 2022 Jun 1; 18(6): e1010241.
  12. Noda T, Blaha A, Fujihara Y, Gert KR, Emori C, Deneke VE, Oura S, Panser K, Lu Y, Berent S, Kodani M, Cabrera-Quio LE, Pauli A, Ikawa M. Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun Biol. 2022 Apr 7;5(1):332.
  13. Morohoshi A, Miyata H, Oyama Y, Oura S, Noda T, and Ikawa M. FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice. Development. 2021 Nov 1;148(21):dev199644.
  14. Miyata H, Oura S, Morohoshi A, Shimada K, Mashiko D, Oyama Y, Kaneda Y, Matsumura T, Abbasi F, Ikawa M. SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc Natl Acad Sci U S A. 2021 Aug 31;118(35):e2106673118.
  15. Oura S, Noda T, Morimura N., Hitoshi S, Nishimasu H., Nagai Y, Nureki O, Ikawa M. Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG. Commun Biol. 2021 Jun 23;4(1):771.
  16. Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro KI, Ikawa M. KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genetics. 2021 May 7;17(5):e1009412.
  17. Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, Matzuk MM, Ikawa M. ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):e2018355118.
  18. Kim CR, Noda T, Kim H, Kim G, Park S, Na Y, Oura S, Shimada K, Bang I, Ahn JY, Kim YR, Oh SK, Choi HJ, Kim JS, Jung I, Lee H, Okada Y, Ikawa M, Baek SH. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis. Cell Rep. 2020 Jul 28;32(4):107950.
  19. Oura S, Kazi S, Savolainen A, Nozawa K, Castañeda J, Yu Z, Miyata H, Matzuk RM, Hansen JN, Wachten D, Matzuk MM, Prunskaite-Hyyryläinen R. Cfap97d1 is important for flagellar axoneme maintenance and male mouse fertility. PLoS Genet. 2020 Aug 12;16(8):e1008954.
  20. Noda T, Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM, Ikawa M. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502.
  21. Nozawa K, Zhang Q, Miyata H, Devlin DJ, Yu Z, Oura S, Koyano T, Matsuyama M, Ikawa M, Matzuk MM. Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male mice. Biol Reprod. 2020 Aug 4;103(2):244-253.
  22. Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, Ikawa M. Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB J. 2020 Apr;34(4):5389-5400.
  23. Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, Ikawa M. Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Mice. Biol Reprod. 2020 Apr 15;102(4):975-983.
  24. Holcomb RJ, Oura S, Nozawa K, Kent K, Yu Z, Robertson MJ, Coarfa C, Matzuk MM, Ikawa M, Garcia TX. The testis-specific serine proteases PRSS44, PRSS46, and PRSS54 are dispensable for male mouse fertility. Biol Reprod. 2020 Feb 12;102(1):84-91.
  25. Fujihara Y, Noda T, Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, Matzuk MM, Ikawa M. Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506.
  26. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, Ikawa M. CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice. Biol Reprod. 2019 Aug 1;101(2):501-511.
  27. Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M. Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity. Biol Reprod. 2019 Apr 1;100(4):1035-1045.
  28. Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. 2019 Feb 26;68(1):25-34.
  29. Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O. Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Sep 21;361(6408):1259-1262.
  30. Tsuchiya H, Oura S. Involvement of MAFB and MAFF in Retinoid-Mediated Suppression of Hepatocellular Carcinoma Invasion. Int J Mol Sci. 2018 May 13;19(5):1450.

Review articles

  1. Oura S, Hamilton JA, and Wu J. Recent Advances in Stem Cell Based Blastocyst Models. Curr Opin Genet Dev. 2023;81:102088.
  2. Oura S, Mori H, and Ikawa M. Genome editing in mice and its application to the study of spermatogenesis. Gene Genome Edi. 2022;3-4:100014.
  3. 大浦聖矢, 伊川正人. ゲノム編集技術と生殖医学研究への応用. Japanese Journal of Reproductive Endocrinology. 2017;22 (1):5-8, 2017 (in Japanese)

Books

  1. 藤堂具紀／監修
    大浦聖矢, 伊川正人. ゲノム編集による病態モデルマウスの作製. 遺伝子治療開発研究ハンドブック 第2版. 2023
  2. 山本卓／企画
    野田大地, 大浦聖矢, 伊川正人. CRISPR/Cas9を用いた疾患モデルマウスの作製 ～不妊モデルマウスを例に～. 医学の歩み. ゲノム編集の未来. 2020;273 (9):774-779
  3. 山本卓，佐久間哲史／編
    野田大地, 大浦聖矢, 伊川正人. gRNA/Cas9複合体を用いたマウスでのゲノム編集. 実験医学別冊 完全版 ゲノム編集実験スタンダード. 2019;251-266

Presentations

  1. Oura S. Wu J. Towards generating pluripotent stem cell-derived human post-implantation embryo models. International Society for Stem Cell Research, Hamburg, Germany, 2024 (poster)
  2. Oura S. Wu J. Towards mimicking human post-implantation embryo structures with pluripotent stem cells. Keystone Symposium Stem Cell Models for Embryology, Pacific Grove, CA, United States, 2024 (poster)
  3. Oura S and Ikawa M. Trim41 is essential for synapsis regulation and male fertility. Research Institute for Microbial Diseases: Progress meeting, Osaka, Japan, 2022 (oral, hybrid)
  4. Oura S and Ikawa M. Identification of meiosis-required genes using CRISPR/Cas9 and their functional analysis. The 44th Annual Meeting of Molecular Biology Society of Japan, Yokohama, Japan, 2021 (oral, hybrid, invited)
  5. Oura S and Ikawa M. Analysis of male meiosis using KO/Tg mice. Program of totipotency:  from decoding to designing, progress meeting, 2021 (oral, online)
  6. Oura S and Ikawa M. Proximal biotin labeling in testicular germ cells. The 1st meeting of Program of totipotency for young scientists, 2021 (oral, online)
  7. Oura S. How biologists are creating life from scratch. Inuyama Casual Academic Discussoin, Aichi, Japan, 2020 (oral, online, invited)
  8. Oura S, Noda T, and Ikawa M. SpCas9-NG mediated genome editing. Advanced Animal Model Support Outcome Presentation Symposium, Siha, Japan, 2020 (poster)
  9. Oura S, Yonggang L, Sakurai N, Noda T and Ikawa M. CRISPR/Cas9 mediated genome editing in mouse zygotes and analysis of fertility-related genes. Gordon Research Conference Germinal Stem Cell Biology, Hong Kong, 2019 (poster, travel grant)
  10. Oura S, Noda T, and Ikawa M. SpCas9-NG mediated genome editing. Cutting-Edge Biological Science and Technology Encounter 2019, Osaka, Japan, 2019 (poster)
  11. Oura S, Yonggang L, Sakurai N, Noda T, and Ikawa M. Production of gene-modified mice using CRISPR/Cas9. Advanced Animal Model Support Outcome Presentation Symposium, Siha, Japan, 2019 (poster)
  12. Oura S, Yonggang L, Sakurai N, Noda T, and Ikawa M. Genome editing in GFP/RFP tagged ES cells and phenotypical analysis in chimeric mice. Advanced Animal Model Support Technical Training Course for young scientists, Nagano, Japan, 2019 (oral)
  13. Oura S, Noda T, Sakurai N, and Ikawa M. CRISPR/Cas9 mediated genome editing in mouse zygotes and analysis of fertility-related genes. The 3rd meeting of Japanese Society for Genome Editing, Hiroshima, Japan, 2018 (poster)
  14. Oura S, Miyata H, Noda T, Shimada K, Matumura T, Morohoshi A, and Ikawa M. Establishing GFP/RFP tagged ES cells and its application for phenotypic analysis of Hydin deficient sperm. The 65th Annual Meeting of Japanese Association for Laboratory Animal Science, Toyama, Japan, 2018 (oral)
  15. Oura S, Noda T, Isotani A, and Ikawa M. Establishing GFP/RFP tagged ES cells for CRISPR/Cas9 mediated gene function analysis in chimeric mice. Interdisciplinary Program for Biomedical Sciences 8th Progress Meeting, Osaka, Japan, 2018 (poser)
  16. Oura S, Noda T, Isotani A, and Ikawa M. Establishing GFP/RFP tagged ES cells for CRISPR/Cas9 mediated gene function analysis in chimeric mice. 4th World Congress of Reproductive Biology, Okinawa, Japan, 2017 (poser)